Journal: International Journal of Molecular Sciences

Article Title: Inhibition of Aberrant α(1,2)-Fucosylation at Ocular Surface Ameliorates Dry Eye Disease

doi: 10.3390/ijms22157863

Figure Lengend Snippet: Effects of 2-D-gal on conjunctival goblet cell and meibomian gland of the eyelid. ( A ) Representative images of periodic acid–Schiff (PAS) staining of the inferior conjunctival fornix (sagittal section; scale bar: 100 μm) and quantification of PAS-stained goblet cell counts in BALB/c mice under desiccating stress with or without 2-D-gal treatment. ( B ) ELISA for conjunctival MUC5AC. ( C ) Representative transillumination meibography images. ( D ) Quantification of the meibomian gland area of the upper and lower eyelids. A circle depicts data (mean ± SD) from a single individual animal. ns: not significant.

Article Snippet: The protein was extracted from the conjunctiva as described above and assayed for the concentration of MUC5AC by using mouse MUC5AC ELISA kit (Novus Biologicals, Centennial, CO, USA).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: Pharmacological research

Article Title: S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome.

doi: 10.1016/j.phrs.2020.105032

Figure Lengend Snippet: Fig. 2. Effects of SAMC on mucus hypersecre- tion induced by LPS in 16HBE cells and mice. (A) MUC5AC protein expression induced by LPS in 16HBE cells stimulated by different concentrations of LPS. (B) SAMC decreased the expression of MUC5AC protein both in vitro and in vivo. (C) SAMC reduced MUC5AC protein in mouse BALF determined by ELISA. (D) SAMC inhibited MUC5AC/ Muc5ac mRNA both in vitro and in vivo. (E) SAMC inhibited MUC5B/ Muc5b mRNA both in vitro and in vivo. Study groups: C: control group, orally treated with the vehicle only; M: model group, LPS was in- stilled into the lungs of mice through a high- pressure syringe; L and H: orally treated with SAMC 20 and 40 mg/kg after the LPS chal- lenge, respectively; P: positive control, NAC dosed at 500 mg/kg; n = 5 per group. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 compared with the model group. The data are represented as means ± SD. All the experiments were re- peated three times.

Article Snippet: Mouse mucin-5 subtype AC (MUC5AC) ELISA kit was purchased from CUSABIO (Wuhan, China).

Techniques: Expressing, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Control, Positive Control

Journal: Pharmacological research

Article Title: S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome.

doi: 10.1016/j.phrs.2020.105032

Figure Lengend Snippet: Fig. 3. Effects of SAMC on murine airway mucins. Sections show staining with AB-PAS (400×) and immunohistochemical staining (400×) of MUC5AC. The airway surface mucins were shown as the blue staining for the AB-PAS positive and brown staining for MUC5AC. Study groups: C: control group, orally treated with the vehicle only; M: model group, LPS was instilled into the lungs of mice through a high-pressure syringe; L and H: orally treated with SAMC at 20 and 40 mg/kg after the LPS challenge, respectively; P: positive control, NAC dosed at 500 mg/kg. n = 5 per group.

Article Snippet: Mouse mucin-5 subtype AC (MUC5AC) ELISA kit was purchased from CUSABIO (Wuhan, China).

Techniques: Staining, Immunohistochemical staining, Control, Positive Control